Kcnq2^em3Frk

CRISPR/cas9 genome editing is used to introduce theT274M (threonine to methionine; ACC to ATG) and T277T (ACC to ACG; silent PAM deleter) mutations into exon 6. The T274M missense variant is a pathogenic mutation found in some patients diagnosed with Early Onset Epileptic Encephalopathy (EOEE). (J:94077)