一条洛X-P介导的诺姆西林筛选 cassette 替换了exon 20到exon 22的部分。这代表了线1,#29。 Western blot 检测确认了没有蛋白质表达,且有缺陷的 ISGylation(来源:J:330459)
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A loxP flanked neomycin selection cassette replaced part of exon 20 to exon 22. This record represents line 1, #29. Western blot analysis confirmed absence of protein and defective protein ISGylation. (J:330459)