设计了一种靶向载体,其在Wntless同源基因(Drosophila的Wls)编码序列的exon 1上游插入了loxP位点,接着是含frt标签的诺卡因抗性基因(neo)区,再接一个loxP位点位于exon 2上游。通过Flp酶介导的重组,去除了neo cassette,使得exon 1的编码序列被标记为可剪接。随后,Cre介导的重组移除了exon 1。(来源:J:260471)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count