CRISPR/Cas9技术在exon 3产生了一个13个碱基的缺失(gctcgtgctcgtg)。qPCR结果显示内耳中mRNA水平显著降低,而 Western blot分析确认了相应蛋白的缺失。(来源:J:330099)
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CRISPR/Cas9 technology generated a 13 bp deletion (gctcgtgctcgtg) in exon 3. qPCR indicates that mRNA levels are dramatically decreased in the cochlea and Western blot analysis confirmed absence of protein. (J:330099)