通过CRISPR/cas9方法,将CAG-loxP-STOP-loxP-Kcnq2(M547V; ATG到GTG)的cDNA-IRES-EGFP-polyA插件以反向转录方式插入第1内含子。随后,通过Cre介导的重组去除了STOP序列。(来源:J:329150)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
Insertion, Nucleotide substitutions
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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