This Vsx2 enhancer was targeted with sgRNAs (GUUAGACCUAGUCAGAACUC and GUCUAGCUUGUGGAUUUCUU) and an ssODN template using CRISPR/Cas9 technology, resulting in a 11,634 bp deletion (chr12:84578824-84590457 GRCm39) that contains evolutionary conserved regions Rr273 and Rr274. (J:328714)