This Vsx2 enhancer was targeted with sgRNAs (AAGAUGGGUGCAUGGUGCGG and AGACUACAUCUAGACGUGCU) and an ssODN template (GCCCATCACTCTGTCCATCGGTCTGACTAAACAGTTTTGAATAAAGATGGGTGCATGGTGGCTAGGGCCTTCACGAGAACCTCAGCTGTGCGGCACCCTTGAGCCATTTGAACTCTCTCA) using CRISPR/Cas9 technology, resulting in a 5,479 bp deletion (chr12:84596238-84601716 GRCm39). (J:328714)