A Lmx1b-binding sites containing Lmx1b enhancer and suppressor region, regulating expression in limbs, was targeted with sgRNAs (targeting TGGTCCCCAGATATTATGG and TTCCCTTTTGAACCTTGCGG) using CRISPR/Cas9 technology, resulting in a deletion. The deleted region contains enhancers Rr263 and Rr265, and suppressor Rr264. (J:312494)