Sequence for Shh enhancer ZRS or MFCS1 (mammals-fish conserved sequence 1), located in intron 5 of Lmbr1, was deleted using an sgRNA (targeting GAACCTCACACATGATCTAT) with CRISPR/Cas9 technology. The deletion spans 1324 bp (chr5:29519495-29520818 (GRCm39)). (J:316049)