CRISPR/Cas9基因编辑技术用于在基因中产生删除。两个sgRNA的切割位点相距122个基对,位于第三外显子。使用该区域的引物进行PCR结果显示,产生了从163到470bp不等的缺失突变小鼠,这反映了不同长度的插入/缺失(indels)。这些小鼠品系在原始参考文献中未单独区分,因此命名中包含#来表示合并数据。来源:(J:242061)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x C3H)F1
Endonuclease-mediated
基因内删除
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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