Gt(ROSA)26Sor^{tm13(Rr209-lacZ/GFP)Amc}

This allele was created in cells containing the Gt(ROSA)26Sortm11(flpo)Amc RMCE allele. The exchange vector used contains the following elements to replace the sequence between the F3 and FRT sites in the RMCE allele: an F3 site, a neomycin resistance gene cassette minus promoter and ATG translation start site, three poly(A) signal sequences, chicken beta-globin locus insulator sequence, Nkx2-2 enhancer Rr209 (450 bp XbaI-SalI fragment), the hsp68 promoter, a lacZ beta-galactosidase and nuclear GFP reporter fusion gene, poly(A) signal sequence and an FRT site. (J:327127)