Tg(Mapt-ECFP*/Venus*)#Ito

The transgene contains the mouse promoter driving neuron-specific expression, a floxed stop cassette, the YCX2.60 coding sequence [an enhanced cyan fluorescent protein with C-terminal 11 amino acids deleted (ECFPDeltaC11 with R27K and K213N mutations, a rat calmodulin DNA fragment (CaM; aa 5-148 with N60D and D78Y mutations [to increase the fluorescence change for small calcium transients] and E127V mutation), a GG linker, a chicken smooth muscle M13 fragment of myosin light chain kinase (with I-to-S mutation in the 20th aa of the sequence), and a circularly permutated Venus enhanced yellow fluorescent protein (cp173 Venus; aa 174-239, a 15 bp linker and aa 1-173)], Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 intron, and polyadenylation sequence. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:270642)