Mrps5^tm1.2Dshc

This allele was created using the FLEx switch system. A loxP site was inserted upstream of exon 10, a lox511 site, inverted exon 10 containing a glycine to arginine substitution at amino acid 315 (p.G315R), an FRT flanked neomycin selection cassette, loxP site, and lox511 site were inserted downstream of exon 10. The neomycin cassette was removed via flp-mediated recombination. Cre-mediated recombination in the germline removed the wild-type exon 10 and flipped the duplicated inverted exon bearing the G315R mutation resulting in expression of the mutant allele. (J:325948)