CRISPR/cas9基因编辑通过两个导向RNA(uORF1 sgRNA: 5'-CCGGCGGGAATTTTTCAAAA-3'和uORF2 sgRNA: 5'-GCGTAGAGAAGGCGTCCTGA-3')以及单链DNA修复模板,实现对目标的修改,具体是将uORF1和uORF2的起始密码子从ATG变为ATA(来源:J:326689)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x SJL)F2
Endonuclease-mediated
核苷酸替换
--
1
4
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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