CRISPR/cas9基因编辑通过两个导向RNA(uORF1 sgRNA: 5'-CCGGCGGGAATTTTTCAAAA-3'和uORF2 sgRNA: 5'-GCGTAGAGAAGGCGTCCTGA-3')以及单链DNA修复模板,实现对目标的修改,具体是将uORF1和uORF2的起始密码子从ATG变为ATA(来源:J:326689)。
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count