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中文
靶向载体被设计用于在Col1a1基因3' UTR下游500bp的位置插入无FRT-介导的PGK-Neo启动子和无ATG的hygromycin抗性基因元件，这个元件带有FRT-flank。随后，Flpe重组酶被用来实现FLP-In操作，将tetO-Plk4-EYFP质粒整合。这个Plk4-EYFP质粒包含了带有EYFP标签的鼠Plk4开放阅读框，位于tetO响应元件（tetO）和CMV微弱启动子之后。(来源：J:272389)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
