CRISPR/Cas9技术通过5'-ggatgcagctaaggctttgg-3'和5'-gacttctggtgacatatctt-3'的gRNAs创建了一个大的片段敲除。 Western blot分析证实了在纯合突变雄性睾丸中蛋白质表达的缺失。(来源:J:322392)
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CRISPR/Cas9 technology using gRNAs 5'-ggatgcagctaaggctttgg-3' and 5'-gacttctggtgacatatctt-3' created a large fragment knockout. Western blot analysis confirmed the absence of protein expression in the testes of homozygous mutant males. (J:322392)