Rfx2^tm1Hncs

Exons 6 and 7, encoding the DNA binding domain, were replaced with a phosphoglycerate kinase-neomycin-resistance cassette using homologous recombination. RT-PCR analysis showed that deletion of exon 6 and 7 results in the splicing of exon 5 to exon 8, leading to a frame shift and premature termination at an out-of-frame stop codon. Western blot analysis confirmed the absence of protein expression in total testis extracts from homozygous mutant mice. (J:230575)