CRISPR/Cas9通过同源定向修复(HDR)技术,在终止密码子前插入mCherry报告基因。使用两种sgRNAs,sgRNA1为ATTCTTATAGAGGACGCACT,sgRNA2为AAGTGCGTCCTCTATAAGAA,或者sgRNA1和sgRNA3的组合(AACATTGTGGTGGCTGATAT),目标定位在第4外显子和3'非编码区的连接处。(来源:J:302430)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Endonuclease-mediated
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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