CRISPR/cas9基因编辑工具被用来在基因的3' UTR末端插入一个含有IRSE(间隔序列)-随后是F3-FRT-Cre-F3-FRT序列,反向排列的元件。这个 cassette 包含大约1kb的5'和5'同源臂序列。Flpo重组酶的表达能将cre定向重组到正确位置。(来源:J:325967)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6 x SJL
Endonuclease-mediated
插入
--
1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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