这个等位基因是在表观基因组学中心通过电穿孔,将Cas9核糖核酸复合物与单链引物RNA配对,其中5'端的序列是GAAAAGCAAATACCGCCGGG,3'端的为AAACATTAAACTAGGCGGTC,目标区域是ENSMUSE00000520605。同时,伴随一个单链Bxb1 attB座的短链核酸。这导致了1292bp的chr11染色体从77,465,754到77,467,045(GRCm39)位置的缺失,插入了一个Bxb1 attB序列(GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCGG)。(来源:J:265051)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6NCrl
Endonuclease-mediated
插入,基因内删除
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1
3
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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