通过CRISPR/Cas9技术,研究人员在16号染色体的Chondrolectin基因(Chodl)目标终止密码子位置插入了P2A-Cre(编码一种能介导核糖体跳过和优化后的Cre基因),以及一个myc标签。通过将胚胎干细胞与囊胚期胚胎融合并繁殖,产生了嵌合小鼠。这个等位基因中还保留了氨苄青霉素载体元件。(来源:J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Endonuclease-mediated
插入
--
1
--
--

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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