靶向载体设计用于替换基因的exon 1区域,替换为完整的人类PRL编码序列,后面跟着一个带有floxed Neo cassette的元件。通过Cre介导的重组, Neo cassette被去除了。免疫印迹分析显示这些小鼠不表达内源性泌乳素。(来源:J:324566)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count