Tg(Met,-lacZ)#Buck

ligonucleotides containing the Pax3 consensus binding site were multimerized. The sequence of the oligonucleotides is as follows: CSSPax3-A, 5-CCTCGTCACGCTTCGAATGT-3; and CSSPax3-B, 5-GGGACATTCGAAGCGTGACGA-3. The oligonucleotides were annealed and polymerized. Concatenamers containing five binding sites were gel-purified, and cloned into a TK-nLacZpA vector, which contains the nlacZ reporter gene under the control of the thymidine kinase (TK) promoter, into an SfiI site located 5 to the TK minimal promoter. A 7.4-kb plasmid fragment for injection was isolated using XhoI and NotI. (J:82809)