Gt(ROSA)26Sor^{tm1(Uprt)Bara}

The cDNA of Uprt from Toxoplasma gondii (T. gondii) was inserted into a Rosa26 targeting vector. A knockin vector was created with Uprt cDNA flanked by a floxed-stop cassette. Uprt-knockin mice were generated by standard homologous recombination in mouse embryonic stem (ES) cells. The presence of transcriptional stop cassette PGK-neo-pA-3xpA prevented downstream Uprt cDNA expression, while mating with mice expressing tissue/cell-specific Cre activated the expression of Uprt cDNA in a cell-specific manner. (J:324326)