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中文
这个crRNA（AAUGAAAGCAGAGCUGCUUC）被设计用于在基因的终止密码子之前插入一个Ddc40HA-V5-P2A-mRuby2-3xNLS构建。具体来说，构建包含了从5'到3'的序列：Ddc编码序列的最后40bp，末端连接一个V5抗原标签，P2A自切割肽序列，三个NLS序列（3xNLS）修饰的mRuby2，终止密码子，37bp变异的Ddc3'非编码区（mUTR）以防止与下游34bp的CRISPR切割位点的同源修复（保留14号外显子的3'剪接接点），然后是40bp未变异的Ddc14内含子序列。来源：(J:324028)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
