这个crRNA(AAUGAAAGCAGAGCUGCUUC)被设计用于在终止密码子之前插入Ddc-6His-P2A-eGFP-3xNLS构建。具体来说,构建包含了从5'到3'的序列:Ddc编码序列的最后40bp,末端连接一个6His标签,P2A自切割肽,三个NLS序列(3xNLS)修饰的EGFP,终止密码子,37bp变异的Ddc3'-UTR(mUTR)以阻止终止密码子与下游34bp的CRISPR切割位点的同源修复(保留14号外显子的3';-剪接接点),以及40bp未变异的Ddc14内含子序列。来源:(J:324028)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
插入
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1
1
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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