Mice were generated by inserting a 2A sequence, mCherry, 2A sequence, and the C terminal 36 amino acids of the DnaE split intein sequence from Nostoc punctiforme (Npu) fused to the C-terminal fragment of tTA (aa 206-248), FLAG tag, and Frt-flanked neomycin resistance cassette immediately in front of the stop codon of Scgb1a1 coding sequence by homologous recombination. The Frt-flanked neomycin resistance cassette was removed by crossing with hACTB-Flpe deleter mice. (J:323339)