通过同源重组,将诺斯科·普金福米翁斯(Nostoc punctiforme)的DnaE分裂 intein序列的C端36个氨基酸(Npu的C末端)、mCherry标记、2A序列以及增强型Cre(iCre)的C端片段(aa 105-343)融合到Scgb1a1基因的终止密码子前方,生成了小鼠。随后,通过与携带hACTB-Flpe删除基因的实验鼠杂交,移除了Frt-flanked neomycin抗性基因 cassette。来源:(J:323339)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
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1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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