设计了一种靶向载体,用于在基因的3'端插入一个双抗原糖(HA)标签。在第6外显子上游插入了LoxP序列,随后是含FRT标记的诺卡因抗性基因座,下游则插入了另一个LoxP位点。通过FLP介导的生殖细胞重组,去除了诺卡因抗性基因座,留下LoxP序列环绕着HA标记的第6外显子。(来源:J:272910)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S6/SvEvTac x C57BL/6)F1
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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