Adgrl2^tm1.1Sud

A targeting vector was designed to introduce a loxP site upstream of the first coding exon (exon 2). A segment carrying an F1 FRT site, FRT site, 1 kb of the mouse engrailed splice acceptor, 5 kb of Adgrl2 cDNA fused to mVenus green fluorescent protein, a polyA signal, FRT site, PGK-neomycin resistance cassette, tandem FRT and F1 FRT sites, and a loxP site were placed in intron 2. Flp-mediated recombination in the germline removed the neomycin selection cassette flanked by F3 sites, leaving the fusion of the cDNA/Venus gene fusion intact and expressed under the control of the endogenous promoter. (J:246190)