CRISPR/Cas9通过指导RNA靶向exon 2的重组产生了2个碱基(TC)的缺失。 Western blot结果显示,来自纯合突变小鼠大脑、卵巢和睾丸的匀浆中均检测不到蛋白质表达。(来源:J:313356, J:323073)
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CRISPR/Cas9 mediated recombination with guide RNAs targeting exon 2 created a 2 bp (TC) deletion. Western blot analysis confirmed the absence of protein expression in brain, ovary and testis lysates from homozygous mutant mice. (J:313356, J:323073)