CRISPR技术插入了一个位于2号外显子上游的loxP位点,以及一个3'带有FRT标记的诺卡因抗性基因座,它位于2号外显子下游。通过Flp介导的重组,去除了选择性基因座。随后,Cre介导的重组剔除了2号外显子。(来源:J:322242)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Targeted
基因内删除
--
1
--
--

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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