Epha2^em1Alsh

Using sgRNAs (targeting GTTCAGTGTACTTCAGTTGGNGG and TTCAGTGTACTTCAGTTGGTNGG) and an SSODN template (GGCCAGGTCCCGGTGCACGTAGTTCATGTTGGCCAGGTACTTCATGCCGGATGCGATACCCTGCAGCATGCCCACTAGCTGAAGTACACTGAACTCACCATCCTTCTCCTGCAGAGATAGGCCCTCAGTGCTGACCGG) with CRISPR/Cas9 technology, arginine codon 722 (AGG) in exon 13 was changed to glutamine (CAG) (p.R722Q). The equivalent p.R721Q human mutation is associated with age-related cortical cataract. (J:312486)