靶向载体设计成通过插入exon 1内,将pH敏感的超扁平pHluorin(SEP)绿色荧光蛋白(GFP)的变体连接到细胞外N端。下游位置插入了含loxP位点的诺卡因抗性(neo)基因座。随后,Cre介导的重组移除了loxP标记的neo基因座。(来源:J:321813)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count