The targeting vector inserted a short linker followed by the coding sequence for mCherry and an FRT (FLP recognition target)-flanked phospho-glycerate kinase (PGK) promoter-driven neomycin (neo) resistance gene after the last codon of Gata1 gene (skipping the endogenous stop-codon). The FRT-flanked neo selection cassette was deleted in vivo by a Flpe deleter strain by recombinase-mediated excision. (J:236311)