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Using an sgRNA (targeting GCAGTTTAATATGACGGAGG in exon 4) and an ssODN template (CCTTGTGATCAGCGATCTCCTTTTTCCTCCTTTCTGAAGGCGAACGCCCGTTCCAGTGTAATCAGTGCGGGGCATCTTTTACTCAGAAACGTAATCTCCTCCGTCATATTAAACTGCACACGGGGGAAAAACCTTTTAAGTGTCACCTCTGCAACTACGCATGCCAAAGGAGAGATGCGCTCACGGGACACCTTAGGACA) with CRISPR/Cas9 technology, a G>C mutation was engineered in glycine codon 158, changing it to arginine (p.G158R), to mimic a mutation (p.G159R) found in human patients with adaptive immunity defects (B cell developmental deficiencies and T cell abnormalities). (J:319672)