CRISPR/cas9基因编辑通过在原设计用于创建条件性敲除突变的外显子4上游(ACTTATACCATCAGTTAGAT; AGCAGGACCAATCTAACTGA)和下游(CTGCTGGCTCATAGTGTGCG; CCCGCACACTATGAGCCAGC)区域使用引导RNA,意外地产生了一个553bp的缺失,包括了整个外显子4(从TCTGCTTAAAGCAGGACCAATCT到/del553/GCGGGGTAGCATTTAAAGGCAGCAC)。(来源:J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
基因内删除
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1
2
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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