CRISPR介导的重组产生了一个覆盖从2到14号外显子的缺失,这一结果通过基因组PCR和 Western blotting 在主动脉平滑肌细胞和心脏内皮细胞中得到了确认(来源:J:319500)
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CRISPR-mediated recombination created a deletion that spanned exons 2 to 14, as confirmed by genomic PCR and Western blotting in aorta smooth muscle cells and heart endothelial cells. (J:319500)