通过CRISPR/Cas9技术,将编码病毒2A寡聚肽(调节 ribosomal 跳过并优化了在哺乳动物细胞中翻译和细胞内及小鼠中重组效率的FLPe基因)的P2A-FlpO序列插入了3号染色体Npnt基因的终止密码子之后。通过将ES细胞与囊胚期胚胎细胞聚合,产生了嵌合小鼠。(来源:J:325783)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Endonuclease-mediated
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部