Gt(ROSA)26Sor^{tm3(CAG-Irs2)Mschu}

The CAG-a loxP site flanked neo-Westphal stop-FLAG-mouse Irs2 cassette followed by an FRT site-framed IRESeGFP expression cassette were inserted into ROSA26 locus via homologous recombination in the ES cells. In presence of cre recombinase, a loxP site flanked neo-Westphal stop sequence is removed to allow expression of FLG epitope-tagged mouse Irs2 driven by CAG promoter. Expression of flp recombinase removes FRT site-framed IRESeGFP. (J:318815)