Prrt2^em1Zqx

CRISPR/Cas9 technology inserted two stop codons (TAGTAA) into the C666 site in exon 2. This mimics the hot-spot mutation at the C649 site of human patients with paroxysmal kinesigenic dyskinesia. Normal levels of transcripts are seen in the brain, however complete loss of protein is seen, indicating a loss-of-function mutation. (J:255335)