CRISPR技术去除了内源性exon 2,并插入了一个带有K71T点突变的exon 2,导致编码的氨基酸从赖氨酸变为丝氨酸,产生了一个名为ha的超精确翻译蛋白。随后,通过Flp酶的重组去除了含FRT标记的neo抗性基因。再通过Cre酶,移除了内源的exon 2。来源:(J:284101)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N
Endonuclease-mediated
Insertion, Single point
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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