Site-specific PhiC31 integrase-mediated transgenesis is used to insert LSL-3X Flag-Map3k12f-T2A-tdTomato into the intergenic site 2 locus (Hipp11). This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice. (J:316717)