A loxP-flanked PGK-neomycin cassette and 3x HA-tagged mouse Vangl2 cDNA containing serine to alanine substitutions at amino acid 5, 82, and 84 (S5A, S82A, S84A) were inserted into the locus. Expression can be induced by cre-mediated excision of the loxP-flanked stop cassette. (J:314584)