A loxP-flanked PGK-neomycin cassette and 3x HA-tagged mouse phospho-mutant Vangl2 cDNA in which all phosphorylation sites identified in two clusters of Vangl2 were mutated to alanines were inserted into the locus. Expression can be induced by cre-mediated excision of the loxP-flanked stop cassette. (J:314584)