在内皮1中插入了一个从5'到3'带有loxP位点的neo-stop cassette、一个rox位点的stop cassette,以及CAGGS启动子驱动的2A-NLS-ZsGreen(鸡β-actin启动子带有CMV增强子)。这些带有Gt(ROSA)26Sortm1(CAG-ZsGreen)Hamm标记的转基因小鼠与CAGGS Dre小鼠杂交,目的是去除rox位点的stop cassette。通过插入的间隔的floxed neo-STOP cassette,ZsGreen的表达仅在cre介导剪切去除了neo-STOP cassette时才得以激活。(来源:J:314807)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
B6.Cg-Thy1a
Targeted
插入
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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