Tg construct was created by modifying mouse BAC clone to insert the nuclear localization signal (NLS) Cre-pA cassette and FRT-Amp-FRT sequence into the translational initiation site of the Prkcd gene. The Amp sequence in the obtained BAC construct was removed by flp/FRT recombination in bacteria. Among the multiple lines obtained, a line (P) with high selectivity of recombination in the dorsal thalamic nuclei was selected. (J:254126)