Fndc5^tm1.1Jkche

Using homologous recombination in ES cells, the targeting vector inserted a loxP upstream of the exon 2 of the Fndc5 gene and FRT flanked Neo selection cassette and the second loxP sequences in the intron downstream of the exon 2. The neo cassette was removed by Flp recombinase, leaving the exon 2 flanked by loxP sites. Cre mediated removal of exon 2 will result in a 75 aa (28 aa Nterminal sequence of irisin and 47 aa frameshifted nonsense aa) truncation. (J:314278)