Themis^tm1.1Ciphe

Sequence for a OneSTrEPtag (OST) and a Gly-Ser-Gly spacer was inserted in-frame at the 5 end of the gene coding sequence. The loxP site flanked mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, cre recombinase gene, and neomycin resistance gene cassette sequence that was also inserted, was removed through Cre-mediated self-excision in the male germline. The fusion of the OST to the protein can be used in affinity purification. (J:282407)