Ptpn22^tm2.1Ciphe

Sequence for a loxP site, a OneSTrEPtag (OST) and a Gly-Ser-Gly spacer was inserted in-frame at the 5 end of the gene coding sequence and a second loxP site in inton 1. The FRT site flanked neomycin resistance gene cassette that was also inserted was removed through subsequent Flp-mediated recombination. The fusion of the OST to the protein can be used in affinity purification. (J:282407)